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May 1, 1993Molecular and Cellular Biology

Functional Analysis of a Stable Transcription Arrest Site in the First Intron of the Murine Adenosine Deaminase Gene

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Key result

The intron 1 arrest site of the murine ADA gene is a stable RNA polymerase II pause site where transcription elongation factor SII promotes read-through.

Population

Murine adenosine deaminase (ADA) gene model

Comparison

Transcription elongation factor SII, specific… vs Wild-type sequence and natural position

Design

Preclinical

Authors

SKShera KashAnsys (United States)JIJeffrey W. InnisUniversity of GenevaAJAnne JacksonAstraZeneca (United Kingdom)

Discussion

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Implication

Caution against clinical translation from murine models; leaves open ADA transcriptional pausing in human cardiovascular disease.

Structured PICO

P
Population
Murine adenosine deaminase (ADA) gene model
I
Intervention
Transcription elongation factor SII, specific point mutations, and sequence position changes
C
Comparator
Wild-type sequence and natural position
O
Outcome
Transcription arrest and pausing efficiencysurrogate

The study characterizes the functional sequence determinants of a stable RNA polymerase II pause site in the first intron of the murine ADA gene.

Cite This Study

Kash et al. (1993) studied this question. Transcription elongation factor SII and specific point mutations was evaluated on Transcription read-through and pausing efficiency at the intron 1 arrest site. The intron 1 arrest site of the murine ADA gene is a stable RNA polymerase II pause site where transcription elongation factor SII promotes read-through.

synapsesocial.com/papers/6a96981b37dc33ab6afbbd68https://doi.org/10.1128/mcb.13.5.2718-2729.1993
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Also Consider

Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Identification of transcription stop sites at the 5' and 3' ends of the murine adenosine deaminase gene.1990 · 44 citations
  2. 2The block to transcriptional elongation within the human c-myc gene is determined in the promoter-proximal region.1992 · 271 citations
  3. 3Elongation factor-dependent transcript shortening by template-engaged RNA polymerase II.1992 · 199 citations
  4. 4Purification and functional characterization of transcription factor SII from calf thymus. Role in RNA polymerase II elongation.1987 · 90 citations