Key result
In the presence of 50% formamide, 84 nucleotides 3' of the break site were required for self-cleavage of hepatitis delta virus genomic RNA, whereas 82 nucleotides were required in its absence.
Population
Precursor RNAs of hepatitis delta virus genomic RNA
Comparison
50% formamide in 2 mM MgCl2 at 37 degrees C vs Absence of formamide
Design
Preclinical
Authors
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Condition-dependent minimal domains for HDV RNA self-cleavage identified; leaves open whether in vivo regulation occurs via alternative structures.
The study defines condition-dependent minimal domains for self-cleavage of hepatitis delta virus genomic RNA, suggesting that cleavage in vivo could be regulated by alternative RNA structures.
Perrotta et al. (1990) studied Hepatitis delta virus. Formamide vs. Absence of formamide was evaluated on Sequence requirements for self-cleavage. In the presence of 50% formamide, 84 nucleotides 3' of the break site were required for self-cleavage of hepatitis delta virus genomic RNA, whereas 82 nucleotides were required in its absence.
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