Key result
EV-71 viral particles of up to 95% purity can be recovered by a single step ion-exchange chromatography using CIM-DEAE monolithic columns with an overall recovery of 55%.
Population
EV-71 infected Rhabdomyosarcoma (RD) cell supernatant
Design
Preclinical
Authors
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May enable scalable EV-71 vaccine antigen prep; leaves open clinical translation and regulatory validation.
A single-step ion-exchange chromatography method using CIM-DEAE monolithic columns can recover EV-71 viral particles with up to 95% purity and 55% recovery, offering a scalable approach for vaccine development.
Szyporta et al. (2014) studied Enterovirus 71 (EV-71) purification. CIM DEAE-8f tube monolithic column purification was evaluated on Virus recovery and purity. EV-71 viral particles of up to 95% purity can be recovered by a single step ion-exchange chromatography using CIM-DEAE monolithic columns with an overall recovery of 55%.
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