Key result
Both ozone and erythropoietin acted as protective agents, reducing the muscular damage induced by ischemia-reperfusion injury in the gastrocnemius muscle of adult male rats.
Why the study?
Skeletal muscle ischemia-reperfusion injury causes serious local and systemic complications, and the comparative protective role of ozone versus erythropoietin following gastrocnemius muscle injury required evaluation.
Does ozone or erythropoietin reduce muscular damage in adult male rats with induced skeletal muscle ischemia-reperfusion injury?
Does ozone or erythropoietin reduce muscular damage in adult male rats with induced skeletal muscle ischemia-reperfusion injury?
In a rat model of skeletal muscle ischemia-reperfusion injury, post-reperfusion treatment with either ozone or erythropoietin reduced muscular damage.
Caution against clinical use; leaves open translation of ozone or erythropoietin protection to human ischemia-reperfusion injury.
Ischemia reperfusion (IR) injury of skeletal muscles is a serious problem because of its local and systemic complications. Previous studies reported that ozone and erythropoietin could alleviate IR effect on several organs. The current research is established to evaluate the possible protective role of ozone versus erythropoietin following IR injury of the gastrocnemius muscle. Fifty rats were equally divided into five groups: I control, II ischemia reperfusion (IR), III post-reperfusion ozone treated, IV post-reperfusion erythropoietin-treated, and V recovering post-reperfusion without treatment groups. The right femoral arteries of all rats were clamped for three hours to induce ischemia then clamps were released to allow reperfusion for two hours. Rats of group II were scarified immediately after reperfusion period. Rats of group III were injected with ozone just after reperfusion for 14 days. Animals of group IV were injected with erythropoietin just after reperfusion for 14 days. Rats of group V rats were kept for 2 weeks following reperfusion without treatment. Blood samples were obtained to estimate lactate dehydrogenase (LDH) and creatine kinase (CK) enzymes. Gastrocnemius muscle was processed for measurement of tissue malondialdehyde (MDA), as well as examination by light and electron microscopes. iNOS and PCNA immunohistochemistry and statistical analysis were applied. The current results indicated that both ozone and erythropoietin could be used as protective agents reducing the muscular damage induced by IR injury.
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Gawish et al. (2022) studied induced skeletal muscle ischemia-reperfusion injury (n=50). Ozone or erythropoietin vs. No treatment (IR control and recovering post-reperfusion groups) was evaluated on muscular damage (LDH, CK, tissue MDA, histological and immunohistochemical changes). Both ozone and erythropoietin acted as protective agents, reducing the muscular damage induced by ischemia-reperfusion injury in the gastrocnemius muscle of adult male rats.
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