Key result
Phorbol 12-myristate 13-acetate (PMA) stimulation of human umbilical vein endothelial cells increased cellular ACE mRNA content 10-fold at 24 hours via a protein kinase C-dependent mechanism.
Population
Human umbilical vein endothelial cells (HUVEC)
Comparison
Phorbol 12-myristate 13-acetate (PMA) (100 ng/ml) vs Unstimulated cells or cells treated with…
Design
Preclinical
Follow-up
up to 24 hours
Authors
Loading...
Suggests PKC modulates endothelial ACE expression; leaves open relevance to cardiovascular disease.
Effect estimate: 10-fold increase
PMA-activated PKC strongly increases ACE mRNA levels and gene transcription in human endothelial cells, suggesting a mechanism for ACE regulation in cardiovascular diseases.
Villard et al. (1998) studied this question. Phorbol 12-myristate 13-acetate (PMA) was evaluated on Cellular ACE mRNA content (10-fold increase). Phorbol 12-myristate 13-acetate (PMA) stimulation of human umbilical vein endothelial cells increased cellular ACE mRNA content 10-fold at 24 hours via a protein kinase C-dependent mechanism.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: