What question did this study set out to answer?

To examine how basic fibroblast growth factor influences calcium channel expression and current density during PC12 cell differentiation, and to test whether tyrosine phosphorylation mediates these changes.

August 1, 1994Journal of Neuroscience Research

Fibroblast growth factor‐induced increases in calcium currents in the PC12 pheochromocytoma cell line are tyrosine phosphorylation dependent

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Authors

SRStanley G. RaneGenentech JPJ.D. PollockPurdue University West Lafayette

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Implication

In vitro study shows basic fibroblast growth factor elevates calcium channel currents in PC12 cells through tyrosine phosphorylation, indicating a regulatory mechanism for neuronal excitability.

Key Points

To examine how basic fibroblast growth factor influences calcium channel expression and current density during PC12 cell differentiation, and to test whether tyrosine phosphorylation mediates these changes.
Cultured rat pheochromocytoma (PC12) cells treated with basic fibroblast growth factor (bFGF) for 7 days.
Performed whole-cell patch-clamp recordings using barium as the charge carrier to evaluate current density normalized to cell membrane surface area.
Assessed pharmacological sensitivity using calcium channel blockers (omega-conotoxin, dihydropyridine) and the tyrosine kinase inhibitor genistein.
Treatment with bFGF for 7 days increased total normalized barium current amplitudes approximately 4-fold.
The primary driver of increased current was a 6-fold elevation in high-threshold voltage-activated omega-conotoxin-sensitive barium current, alongside smaller increases in dihydropyridine-sensitive and insensitive currents.
Co-treatment with the tyrosine kinase inhibitor genistein completely blocked the bFGF-induced increases in barium currents.

Cite This Study

Rane et al. (1994) studied this question.

synapsesocial.com/papers/6a96d3ce17cb0e8b160e3234 https://doi.org/10.1002/jnr.490380511

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Implication

In vitro study shows basic fibroblast growth factor elevates calcium channel currents in PC12 cells through tyrosine phosphorylation, indicating a regulatory mechanism for neuronal excitability.

Fibroblast growth factor‐induced increases in calcium currents in the PC12 pheochromocytoma cell line are tyrosine phosphorylation dependent

Discussion

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Implication

Key Points

Cite This Study

Fibroblast growth factor‐induced increases in calcium currents in the PC12 pheochromocytoma cell line are tyrosine phosphorylation dependent

Discussion

Member takes

Implication

Key Points

Cite This Study

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