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Virus-specific RNA is present in polyribosomes of transformed cells, with 60 to 70% released by EDTA, consistent with its function as messenger RNA for viral-coded proteins.
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We present evidence that virus-specific RNA is present in polyribosomes of transformed cells replicating the murine sarcoma-leukemia virus complex and that it serves as messenger RNA for the synthesis of viral-coded proteins. Both virus-specific RNA (detected by hybridization with the [(3)H]DNA product of the viral RNA-directed DNA polymerase) and nascent viral polypeptides (measured by precipitation with antiserum to purified virus) were found in membrane-bound and free polyribosomes. Membrane-bound polyribosomes contained a higher content of both virus-specific RNA and nascent viral polypeptides. From 60 to 70% of viral RNA sequences were released from polyribosomes with EDTA, consistent with a function as messenger RNA. Maximum amounts of both virus-specific RNA and nascent viral polypeptides were found in the polyribosome region sedimenting at about 350 S.
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Vecchio et al. (1973) studied Murine sarcoma-leukemia virus replication. Virus-specific RNA is present in polyribosomes of transformed cells, with 60 to 70% released by EDTA, consistent with its function as messenger RNA for viral-coded proteins.
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