What question did this study set out to answer?

To assess whether the suppression of plant tumor formation by Agrobacterium tumefaciens at temperatures above 29 °C stems from a defect in the T-DNA transfer machinery.

March 1, 1996Journal of BacteriologyOpen Access

Temperature affects the T-DNA transfer machinery of Agrobacterium tumefaciens

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To assess whether the suppression of plant tumor formation by Agrobacterium tumefaciens at temperatures above 29 °C stems from a defect in the T-DNA transfer machinery.
Tested the mobilization of an incompatibility group Q (IncQ) plasmid mediated by the A. tumefaciens T-DNA transfer apparatus at multiple incubation temperatures.
Evaluated the requirement for plasmid mobilization upon chemical induction with acetosyringone (for virB and virD operons) versus octopine (for tra genes), and tested mobilization under alternative conjugation machineries.
Plasmid transfer reached optimal efficiency at 19 °C and was completely blocked at incubation temperatures exceeding 28 °C.
Transfer required induction of the virB and virD operons by acetosyringone, but temperature-dependent variations in vir induction levels did not explain the loss of transfer at higher temperatures.
Alternative transfer systems successfully mobilized the IncQ plasmid at elevated temperatures, confirming that high temperature selectively impairs the A. tumefaciens T-DNA transfer machinery itself.

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Implication

Experimental study reveals temperature-dependent failure of T-DNA transfer in Agrobacterium tumefaciens, suggesting heat directly impairs the transfer apparatus rather than gene induction.

Cite This Study

Fullner et al. (1996) studied this question.

synapsesocial.com/papers/6a96e33f23945b24488243e5 https://doi.org/10.1128/jb.178.6.1498-1504.1996

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Implication

Experimental study reveals temperature-dependent failure of T-DNA transfer in Agrobacterium tumefaciens, suggesting heat directly impairs the transfer apparatus rather than gene induction.

Temperature affects the T-DNA transfer machinery of Agrobacterium tumefaciens

Key Points

Discussion

Implication

Cite This Study

Temperature affects the T-DNA transfer machinery of Agrobacterium tumefaciens

Key Points

Discussion

Implication

Cite This Study

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