What question did this study set out to answer?

To systematically evaluate the effect of temperature on the efficiency of Agrobacterium tumefaciens-mediated T-DNA transfer to plant tissues.

December 1, 1997The Plant JournalOpen Access

The effect of temperature on Agrobacterium tumefaciens‐mediated gene transfer to plants

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To systematically evaluate the effect of temperature on the efficiency of Agrobacterium tumefaciens-mediated T-DNA transfer to plant tissues.
Tested Agrobacterium tumefaciens strains carrying a binary vector with the beta-glucuronidase (uidA) gene and nopaline-, octopine-, or agropine/succinamopine-type helper plasmids.
Assayed transient gene transfer efficiency across temperatures ranging from 15°C to 29°C.
Utilized two transformation models: Phaseolus acutifolius callus cocultivation and Nicotiana tabacum leaf vacuum-infiltration.
Transient uidA expression decreased markedly in both plant systems when temperatures exceeded 22°C, irrespective of the helper plasmid type.
Gene expression was notably low at 27°C and completely undetectable at 29°C.

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Implication

Experimental study demonstrates that temperatures above 22°C suppress Agrobacterium-mediated gene transfer in plants, indicating that standard transformation protocols require temperature...

Cite This Study

Dillen et al. (1997) studied this question.

synapsesocial.com/papers/6a9f6e3aff2e3aa83ec07728 https://doi.org/10.1046/j.1365-313x.1997.12061459.x

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Implication

Experimental study demonstrates that temperatures above 22°C suppress Agrobacterium-mediated gene transfer in plants, indicating that standard transformation protocols require temperature...

The effect of temperature on Agrobacterium tumefaciens‐mediated gene transfer to plants

Key Points

Discussion

Implication

Cite This Study

The effect of temperature on Agrobacterium tumefaciens‐mediated gene transfer to plants

Key Points

Discussion

Implication

Cite This Study

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