Key result
PCR assays in the presence of antibodies to thermostable DNA polymerases detected 10- to 100-fold lower titer specimens of Influenza A and 10-fold lower titer specimens of Adenovirus compared to assays without antibodies.
Why the study?
Does the presence of antibody to thermostable DNA polymerases improve the sensitivity of PCR for detecting low-titer Influenza A and Adeno virus specimens?
Does the presence of antibody to thermostable DNA polymerases improve the sensitivity of PCR for detecting low-titer Influenza A and Adeno virus specimens?
Effect estimate: 10- to 100-fold lower titer detection for Influenza A and 10-fold for Adenovirus
The addition of antibodies to thermostable DNA polymerases in PCR assays significantly increases the sensitivity for detecting low-titer viral specimens.
May enhance PCR sensitivity for low-titer viruses in research; leaves open clinical translation pending validation.
The serial low-titer specimens of Influenza A virus and Adeno virus type 7 were tested for the presence of virus specific genes by PCR based on Tth DNA polymerase and by that based on Taq DNA polymerase, in the absence and presence of antibody to the respective DNA polymerases. Increased product DNA synthesis and higher sensitivity of detection were observed in the presence of antibody compared to those in the absence of antibody. 10- to 100- fold lower titer specimen of Influenza A virus and 10-fold lower titer specimen of Adeno virus could be detected in the presence of antibody than those detected in the absence of antibody to the appropriate DNA polymerase, in a PCR.
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Poddar et al. (1998) studied Influenza A virus and Adeno virus type 7 detection. Antibody to thermostable DNA polymerases (Tth and Taq) vs. Absence of antibody was evaluated on Detection of virus specific genes by PCR (10- to 100-fold lower titer detection for Influenza A and 10-fold for Adenovirus). PCR assays in the presence of antibodies to thermostable DNA polymerases detected 10- to 100-fold lower titer specimens of Influenza A and 10-fold lower titer specimens of Adenovirus compared to assays without antibodies.
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