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October 1, 1996Journal of Clinical MicrobiologyOpen Access

Comparison of reverse transcription-PCR with tissue culture and other rapid diagnostic assays for detection of type A influenza virus

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Why the study?

Does RT-PCR improve diagnostic accuracy for influenza A virus compared to virus cultivation and rapid diagnostic kits in pediatric outpatients with acute respiratory illness?

Population

Outpatient pediatric population with acute respiratory illness during concurrent epidemics of influenza A…

Comparison

Reverse transcription-PCR assay for influenza A… vs Virus cultivation and commercially available…

Design

Cross-sectional

Authors

RARobert L. AtmarBaylor College of MedicineBBBarbara D. BaxterJohn A. Logan CollegeEDE A DominguezUniversity of Nebraska at Omaha

Discussion

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Implication

RT-PCR may offer higher sensitivity than rapid kits in pediatric outpatients; leaves open whether routine adoption improves clinical outcomes.

Structured PICO

Does RT-PCR improve diagnostic accuracy for influenza A virus compared to virus cultivation and rapid diagnostic kits in pediatric outpatients with acute respiratory illness?

P
Population
Outpatient pediatric population with acute respiratory illness during concurrent epidemics of influenza A virus and respiratory syncytial virus
I
Intervention
Reverse transcription (RT)-PCR assay for influenza A virus on combined nasal wash-throat swab specimens
C
Comparator
Virus cultivation (gold standard) and commercially available rapid diagnostic kits (e.g., Becton Dickinson Directigen)
O
Outcome
Diagnostic sensitivity, specificity, and efficiencysurrogate

RT-PCR is an effective and highly sensitive alternative to virus isolation for detecting influenza A virus in clinical specimens.

Cite This Study

Atmar et al. (1996) studied this question.

synapsesocial.com/papers/6a77fa8da7498e5110af076bhttps://doi.org/10.1128/jcm.34.10.2604-2606.1996
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Detection of Influenza Viruses in Throat Swab by Using Polymerase Chain Reaction1991 · 54 citations
  2. 2Detection of enteric viruses in oysters by using the polymerase chain reaction1993 · 193 citations
  3. 3Use of PCR-enzyme immunoassay for identification of influenza A virus matrix RNA in clinical samples negative for cultivable virus1994 · 76 citations
  4. 4Detection of influenza A and B in respiratory secretions with the polymerase chain reaction.1992 · 54 citations
  5. 5Comparison of rapid diagnostic techniques for respiratory syncytial and influenza A virus respiratory infections in young children1993 · 101 citations