Why the study?
Does nested RT-PCR improve the detection of influenza viruses A and B compared to virus isolation and immunofluorescence in patients with suspected infection?
Population
215 nasopharyngeal aspirates from patients with suspected influenza virus infection, and 105 nasopharyngeal…
Comparison
Nested reverse transcription-PCR and optical… vs Virus isolation and direct immunofluorescence…
Design
Other
Authors
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Supports RT-PCR for enhanced influenza detection; leaves open OIA's clinical role pending specimen-specific validation.
Does nested RT-PCR improve the detection of influenza viruses A and B compared to virus isolation and immunofluorescence in patients with suspected infection?
Nested RT-PCR provides a more sensitive method for detecting influenza A and B compared to traditional virus isolation and immunofluorescence, while rapid OIA requires further evaluation due to variable sensitivity.
Herrmann et al. (2001) studied this question.
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