Why the study?
Does the optical immunoassay test (FLU OIA) accurately detect influenza A and B viruses compared to viral isolation in pediatric patients with acute respiratory illnesses?
Does the optical immunoassay test (FLU OIA) accurately detect influenza A and B viruses compared to viral isolation in pediatric patients with acute respiratory illnesses?
The FLU OIA rapid test demonstrates moderate to high sensitivity and specificity for detecting influenza viruses, performing better with nasopharyngeal aspirates than throat swabs.
Sample-dependent accuracy cautions against sole reliance for pediatric influenza diagnosis; leaves open need for prospective validation of rapid tests.
The optical immunoassay test (FLU OIA, BioStar, USA) for rapid detection of influenza A and B viral antigens was compared with viral isolation in cell culture. A total of 103 respiratory specimens were tested on 75 pediatric patients with acute respiratory illnesses. Influenza viruses were recovered in 40 specimens (type A: 5, Type B: 35). FLU OIA demonstrated 80.0% sensitivity and 68.8% specificity for nasopharyngeal aspirates and 36.7% sensitivity and 83.9% specificity for throat swabs. We also tested FLU OIA, retrospectively, using 78 supernatant samples from pediatric patients with influenza A virus infection frozen after cell culture. FLU OIA demonstrated 91.4% sensitivity and 92.3% specificity for nasopharyngeal aspirates and 50.0% sensitivity and 91.7% specificity for throat swabs diluted in viral transport media. Nasopharyngeal aspirates showed higher sensitivity than throat swabs for detection of influenza virus by FLU OIA. We believe this rapid test kit is useful for the detection of influenza A and B viruses.
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Sugaya et al. (1999) studied this question.
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