Key result
The R2 reverse transcriptase performs end-to-end template jumps by adding nontemplated nucleotides to the cDNA and annealing them to the 3'-end of the acceptor RNA.
Population
R2 reverse transcriptase and RNA templates (in vitro model)
Design
Preclinical
Authors
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Elucidates R2 RT template-jumping mechanism; leaves open relevance to human retrotransposition or antiviral strategies.
The study describes the mechanism of end-to-end template jumping by the R2 reverse transcriptase, explaining chimeric integration products derived from multiple RNA templates.
Bibiłło et al. (2004) studied this question. R2 reverse transcriptase was evaluated on Mechanism of end-to-end template jumps. The R2 reverse transcriptase performs end-to-end template jumps by adding nontemplated nucleotides to the cDNA and annealing them to the 3'-end of the acceptor RNA.
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