We describe an improved cresolphthalein complexone procedure for calcium in which diethylamine is replaced by a nontoxic amino alcohol. Advantages of the procedure are increased sensitivity, excellent baseline stability, a more optimum reaction environment (pH 10.0) that eliminates blanking problems, and freedom from interference by magnesium. Values obtained by this method are not significantly different (P < 0.05) from those obtained by atomic absorption spectrophotometry.
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Moorehead et al. (1974) studied this question.