August 1, 1998British Journal of PharmacologyOpen Access

2′,3′‐O‐(2,4,6‐ trinitrophenyl) adenosine 5′‐triphosphate (TNP‐ATP)–a nanomolar affinity antagonist at rat mesenteric artery P2X receptor ion channels

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Population

Acutely dissociated rat mesenteric artery smooth muscle cells and whole artery rings

Design

Preclinical

Authors

CLCarolyn J. LewisUniversity of Leicester ASAnnmarie SurprenantUniversity of Manchester RERichard J. EvansWellington Hospital

Discussion

Member takes

Overview

May limit TNP-ATP utility in intact tissues; leaves open stable P2X antagonists for research.

Structured PICO

Population

Acutely dissociated rat mesenteric artery smooth muscle cells and whole artery rings

Intervention

TNP-ATP (2′,3′‐O‐(2,4,6‐ trinitrophenyl) adenosine 5′‐triphosphate)

Outcome

Inhibition of P2X receptor mediated inward currents and contractions induced by alpha,beta-meATPsurrogate

While TNP-ATP is a potent P2X receptor antagonist in isolated cells, its rapid breakdown by nucleotidases limits its utility in whole tissue experiments.

Limitations

TNP-ATP is broken down by nucleotidases in whole tissue experiments
TNP-ATP had non-specific effects on smooth muscle contraction

Cite This Study

Lewis et al. (1998) studied this question.

synapsesocial.com/papers/6a9701d5f69cd3f6920225e2 https://doi.org/10.1038/sj.bjp.0702001

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August 1, 1998British Journal of PharmacologyOpen Access

2′,3′‐O‐(2,4,6‐ trinitrophenyl) adenosine 5′‐triphosphate (TNP‐ATP)–a nanomolar affinity antagonist at rat mesenteric artery P2X receptor ion channels

View Full Paper

Ask AI

Bookmark

Population

Acutely dissociated rat mesenteric artery smooth muscle cells and whole artery rings

Design

Preclinical

Authors

CLCarolyn J. LewisUniversity of Leicester ASAnnmarie SurprenantUniversity of Manchester RERichard J. EvansWellington Hospital

Discussion

Member takes

Overview

May limit TNP-ATP utility in intact tissues; leaves open stable P2X antagonists for research.

Structured PICO

Population

Acutely dissociated rat mesenteric artery smooth muscle cells and whole artery rings

Intervention

TNP-ATP (2′,3′‐O‐(2,4,6‐ trinitrophenyl) adenosine 5′‐triphosphate)

Outcome

Inhibition of P2X receptor mediated inward currents and contractions induced by alpha,beta-meATPsurrogate

While TNP-ATP is a potent P2X receptor antagonist in isolated cells, its rapid breakdown by nucleotidases limits its utility in whole tissue experiments.

Limitations

TNP-ATP is broken down by nucleotidases in whole tissue experiments
TNP-ATP had non-specific effects on smooth muscle contraction

Cite This Study

Lewis et al. (1998) studied this question.

synapsesocial.com/papers/6a9701d5f69cd3f6920225e2 https://doi.org/10.1038/sj.bjp.0702001

Also Consider

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