Single, long columns (1–5 m) can be prepared efficiently using reversed phase packings (3–10 μm particle diameter). 1‐m columns packed with 3 and 10 μm packing provide 110 000 and 50 000 theoretical plates, respectively. Very efficient columns can resolve highly complex mixtures and difficult‐to‐separate compounds. Temperature gradient elution is a powerful technique for LC with microbore columns.
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Hirata et al. (1983) studied this question.