What question did this study set out to answer?

To quantitatively compare the mutagenicity, cytotoxicity, and DNA-binding interactions of reactive polycyclic hydrocarbon derivatives in mammalian cell culture.

August 15, 1979International Journal of Cancer

A quantitative comparison of the mutagenicity of carcinogenic polycyclic hydrocarbon derivatives in cultured mammalian cells

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Authors

RNRobert F. NewboldBrunel University of London PBP. BrookesThe Wistar Institute RHRonald G. HarveyThe Wistar Institute

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Implication

In vitro comparative study demonstrates potent mutagenicity of anti-benzo(a)pyrene diolepoxide in hamster cells, suggesting carbonium ion reactivity drives cellular genetic damage.

Key Points

To quantitatively compare the mutagenicity, cytotoxicity, and DNA-binding interactions of reactive polycyclic hydrocarbon derivatives in mammalian cell culture.
Cultured Chinese hamster (V79) cells exposed to reactive polycyclic hydrocarbon derivatives, measuring mutation frequency via resistance to 8-azaguanine.
Assessed compound activity across applied dose (mutagenic effectiveness), induced cytotoxicity (mutagenic efficiency), and extent of DNA binding (absolute mutagenic efficiency).
Anti-benzo(alpha)pyrene-7,8 dihydrodiol-9,10 oxide exhibited the highest potency among all tested derivatives across all mutagenic measures.
Mutagenicity between syn- and anti-isomer diolepoxides correlated directly with their capacity to form reactive carbonium ions.

Cite This Study

Newbold et al. (1979) studied this question.

synapsesocial.com/papers/6a9755abcfa25b768ec60a25 https://doi.org/10.1002/ijc.2910240212

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Implication

In vitro comparative study demonstrates potent mutagenicity of anti-benzo(a)pyrene diolepoxide in hamster cells, suggesting carbonium ion reactivity drives cellular genetic damage.

A quantitative comparison of the mutagenicity of carcinogenic polycyclic hydrocarbon derivatives in cultured mammalian cells

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Member takes

Implication

Key Points

Cite This Study

A quantitative comparison of the mutagenicity of carcinogenic polycyclic hydrocarbon derivatives in cultured mammalian cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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