The Specificity of Antisera to Conjugates of Prostaglandins E with Bovine Serum Albumin and Thyroglobulin

Sera to conjugates of prostaglandins E1 and E2 with bovine serum albumin and bovine thyro-globulin were raised in rabbits to determine their specificity for these prostaglandins. Antisera to bovine serum albumin conjugates of prostaglandins E1 and E2 were more reactive against prostaglandins A and B respectively. In contrast, the sera prepared with conjugates of thyroglobulin with prostaglandins E1 and E2 displayed marked specificity for the homologous antigens and only minimal cross-reactivity with prostaglandins A and B respectively. These latter antisera were used to develop a sensitive radioimmunoassay for the E prostaglandins.