What question did this study set out to answer?

To develop and validate a highly specific and sensitive radioimmunoassay capable of accurately measuring progesterone directly in crude plasma extracts.

September 1, 1972The Journal of Clinical Endocrinology & Metabolism

A Specific Radioimmunoassay of Plasma Progesterone1

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Authors

GDGUILLERMO O. DEVILLAUniversity of Pennsylvania KRKenneth D. RobertsUniversity of Liverpool WWWalter G. WiestUniversity of London

Discussion

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Implication

Laboratory validation study reveals highly accurate radioimmunoassay of progesterone in human plasma, highlighting a reliable tool for assessing reproductive status.

Key Points

To develop and validate a highly specific and sensitive radioimmunoassay capable of accurately measuring progesterone directly in crude plasma extracts.
Generated a progesterone-specific antibody by immunizing a ewe with 11α-hydroxy-progesterone-hemisuccinate conjugated to bovine serum albumin.
Assayed crude plasma extracts to assess assay sensitivity, recovery accuracy of added progesterone, intra- and inter-assay precision, and steroid cross-reactivity with and without chromatography.
The radioimmunoassay achieved a sensitivity limit of 0.05 ng with intra- and inter-assay coefficients of variation of 8%, showing negligible cross-reactivity from other circulating steroids.
Mean plasma progesterone concentrations were 0.26 ± 0.08 ng/ml in males and postmenopausal females, 0.50 ± 0.44 ng/ml in preovulatory females, and 12.1 ± 4.81 ng/ml in postovulatory females.

Cite This Study

DEVILLA et al. (1972) studied this question.

synapsesocial.com/papers/6a8934684c30e1095aec6102 https://doi.org/10.1210/jcem-35-3-458

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September 1, 1972The Journal of Clinical Endocrinology & Metabolism

A Specific Radioimmunoassay of Plasma Progesterone1

View Full Paper

Ask AI

Bookmark

Authors

GDGUILLERMO O. DEVILLAUniversity of Pennsylvania KRKenneth D. RobertsUniversity of Liverpool WWWalter G. WiestUniversity of London

Discussion

Member takes

Implication

Laboratory validation study reveals highly accurate radioimmunoassay of progesterone in human plasma, highlighting a reliable tool for assessing reproductive status.

Key Points

To develop and validate a highly specific and sensitive radioimmunoassay capable of accurately measuring progesterone directly in crude plasma extracts.
Generated a progesterone-specific antibody by immunizing a ewe with 11α-hydroxy-progesterone-hemisuccinate conjugated to bovine serum albumin.
Assayed crude plasma extracts to assess assay sensitivity, recovery accuracy of added progesterone, intra- and inter-assay precision, and steroid cross-reactivity with and without chromatography.
The radioimmunoassay achieved a sensitivity limit of 0.05 ng with intra- and inter-assay coefficients of variation of 8%, showing negligible cross-reactivity from other circulating steroids.
Mean plasma progesterone concentrations were 0.26 ± 0.08 ng/ml in males and postmenopausal females, 0.50 ± 0.44 ng/ml in preovulatory females, and 12.1 ± 4.81 ng/ml in postovulatory females.

Cite This Study

DEVILLA et al. (1972) studied this question.

synapsesocial.com/papers/6a8934684c30e1095aec6102 https://doi.org/10.1210/jcem-35-3-458

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