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Evaluates serum progesterone measurement in ruminants, indicating reliability of the ELFA method.
BACKGROUND: In ruminants, information on serum progesterone concentrations can be important in monitoring the oestrous cycle for pregnancy testing and the detection of endocrine disorders. We aimed to evaluate the suitability of a commercial enzyme-linked fluorescence assay (ELFA), the MINI VIDAS (bioMérieux, Nürtingen), for measuring progesterone in ovine, caprine and bovine serum samples. METHODS: Blood samples were taken from 391 animals and serum was prepared. Comparative measurements were carried out using ELFA and an in-house radioimmunoassay, which was used as a reference. RESULTS: The results showed a strong positive correlation between the two measurement methods (Spearman rank correlation-cattle: rs = 0.9296 [p < 0.05], sheep: rs = 0.9017 [p < 0.05] and goats: rs = 0.8444 [p < 0.05]). LIMITATIONS: Most samples were clustered at low progesterone concentrations, and potential cross-reactivity with progesterone metabolites was not characterised. CONCLUSIONS: These findings indicate that the commercial ELFA provides a reliable method for determining progesterone concentrations in the studied ruminant species.
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Längerer et al. (2026) studied this question.
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