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The aim is to evaluate a rapid method for measuring serum progesterone in mares to aid in diagnosing estrus cycle issues.

February 8, 2026Open Access

Evaluation of a non-radioactive rapid test for the determination of serum progesterone in the mare

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Evaluates a non-radioactive test for serum progesterone, suggesting a quick diagnostic tool for equine estrus cycle disorders.

The aim is to evaluate a rapid method for measuring serum progesterone in mares to aid in diagnosing estrus cycle issues.
Collected serum samples from 107 mares, divided into two aliquots.
Used enzyme-linked fluorescent assay (mini VIDAS) and radioimmunoassay (RIA) for progesterone measurement.
Performed comparative analysis to assess correlation between methods.
Found a strong positive correlation (rs = 0.96381, p < 0.05) between mini VIDAS and RIA results.
Confirmed mini VIDAS can rapidly determine equine progesterone levels in under one hour.
Identified deviations from RIA in the low measurement range of mini VIDAS, indicating potential limitations.

Längerer et al. (2025) studied this question.

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