July 1, 1991Proceedings of the National Academy of SciencesOpen Access

RNA-protein complexes mediate in vitro capping of the spliced-leader primary transcript and U-RNAs in Trypanosoma cruzi.

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Structured PICO

Population

Trypanosoma cruzi epimastigotes (nuclear extracts)

Intervention

In vitro capping reactions and biochemical fractionation (velocity gradient sedimentation, Cs2SO4 density gradient fractionation, gel filtration chromatography)

Outcome

Characterization of guanylyltransferase (capping) activity and its association with SL-RNA and U-RNAs in ribonucleoprotein particlessurrogate

The study demonstrates that mRNA capping in Trypanosoma cruzi is mediated by RNA-protein complexes (snRNPs) containing guanylyltransferase activity and substrate RNAs, supporting the hypothesis that trans-splicing functions for mRNA capping.

Discussion

No takes yet. Share an insight, caveat, or question.

Implication

Does not yet alter Chagas cardiomyopathy care; extends molecular basis for trans-splicing-dependent capping in T. cruzi.

Cite This Study

Zwierzynski et al. (1991) studied this question.

synapsesocial.com/papers/6a975e3555a55e94cce32dd8 https://doi.org/10.1073/pnas.88.13.5626

View Full Paper

Ask AI

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RNA-protein complexes mediate in vitro capping of the spliced-leader primary transcript and U-RNAs in Trypanosoma cruzi.

Structured PICO

Discussion

Implication

Cite This Study

RNA-protein complexes mediate in vitro capping of the spliced-leader primary transcript and U-RNAs in Trypanosoma cruzi.

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider