Key result
In a human ex vivo model, granulocytes selectively accumulated at the luminal surface of platelet thrombi, independent of fibrin formation but correlated to thrombus growth and platelet activation.
Warrants in vivo validation of granulocyte-thrombus interactions; leaves open clinical relevance and therapeutic targeting.
Since the role of leukocytes found present in thrombi and haemostatic plugs is not clearly understood. we have investigated the interaction between leukocytes and growing thrombi in a human ex vivo model of arterial thrombogenesis. At a wall shear rate characteristic of moderately stenosed arteries (2600 s(-1)), granulocytes selectively accumulated at the luminal surface of platelet thrombi. The leukocyte adhesion seemed independent of fibrin formation and was clearly correlated to thrombus growth and platelet activation. In contrast, flow cytometry revealed that the expression of adhesion molecules (CD11a, CD11b, CD11, CD3, CD14, CD62L, HLA-DR and binding of fibrinogen) on the surface of circulating leukocytes passing the thrombi was, on short term conditions (15 min), independent of thrombus growth. The adhered granulocytes probably play a pivotal role in limiting the size of the evolving thrombi, as suggested by our electron micrographs of the arterial thrombi showing lysed and phagocytosed platelets. Thus, granulocytes might play an active role in the acute/semiacute phase of local thromboregulation.
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Roald et al. (1998) studied Arterial thrombogenesis. Arterial thrombogenesis was evaluated on Leukocyte adhesion and interaction with growing thrombi. In a human ex vivo model, granulocytes selectively accumulated at the luminal surface of platelet thrombi, independent of fibrin formation but correlated to thrombus growth and platelet activation.
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