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April 27, 2022Frontiers in EndocrinologyOpen Access

Effect of lncRNA MALAT1 on the Granulosa Cell Proliferation and Pregnancy Outcome in Patients With PCOS

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Why the study?

Whether long noncoding RNA is involved in anti-Mullerian hormone's role in follicular development in polycystic ovary syndrome is unknown.

Does lncRNA MALAT1 expression affect granulosa cell proliferation and predict pregnancy outcomes in patients with PCOS?

Population

Ovarian granulosa cells from women with and without PCOS, and KGN cells in vitro

Comparison

Women with PCOS vs without PCOS, and MALAT1 knockdown vs control

Design

In vitro laboratory and translational cohort study

Key result

Upregulated lncRNA MALAT1 in granulosa cells of patients with PCOS predicted positive clinical pregnancy outcomes with an area under the curve of 0.771.

Authors

MTMixue TuYWYiqing WuFWFeixia Wang

Discussion

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Overview

AMH upregulates MALAT1 to inhibit granulosa cell proliferation in PCOS; leaves open its role as biomarker or therapeutic target.

Study Design

Type

Case-Control (n=133)

Multicenter

No

Structured PICO

Does lncRNA MALAT1 expression affect granulosa cell proliferation and predict pregnancy outcomes in patients with PCOS?

P
Population
133 women under 40 years old undergoing IVF or ICSI, including 68 with PCOS and 65 controls, evaluated for lncRNA MALAT1 expression and pregnancy outcomes.
E
Exposure
Knockdown of MALAT1; exogenous AMH stimulation
C
Comparator
Women without PCOS; untreated cells
O
Outcome
lncRNA expression, granulosa cell proliferation, and prediction of clinical pregnancy outcomesurrogate

Main Result

Effect estimate: AUC 0.771 (95% CI 0.617-0.925)

p-value: p=0.007

lncRNA MALAT1 is upregulated in PCOS, inhibits granulosa cell proliferation, and may serve as a predictor for clinical pregnancy outcomes.

Limitations

  • Lack of detailed analysis about the expression of MALAT1 in different clinical sample subtypes
  • Lack of expression data of MALAT1 in serum of women with PCOS, which could be a biomarker for diagnosis
  • Unclear whether AMH acts directly on the expression of MALAT1 and how the SMAD signaling pathway acts on the promoter site
  • Needs more molecular mechanism investigation to explain how MALAT1 regulates target gene expression and destroys granulosa cell functions

Cite This Study

Tu et al. (2022) conducted a case-control in Polycystic ovary syndrome (PCOS) (n=133). lncRNA MALAT1 expression vs. Controls without PCOS was evaluated on Prediction of clinical pregnancy outcome (AUC 0.771, 95% CI 0.617-0.925, p=0.007). Upregulated lncRNA MALAT1 in granulosa cells of patients with PCOS predicted positive clinical pregnancy outcomes with an area under the curve of 0.771.

synapsesocial.com/papers/6a977b8f275b4863ba2d1a94https://doi.org/10.3389/fendo.2022.825431
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