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March 1, 1999Journal of the American Society of NephrologyOpen Access

cGMP-Dependent and -Independent Inhibition of a K+ Conductance by Natriuretic Peptides

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Key result

Natriuretic peptides regulate electrogenic transport processes via cGMP-dependent (ANP, BNP, URO) and cGMP-independent (CNP) pathways that depolarize the membrane voltage of human kidney epithelial cells.

Population

Immortalized human kidney epithelial (IHKE-1) cells derived from proximal tubules

Comparison

Natriuretic peptides vs Baseline/control conditions

Design

Preclinical

Authors

JHJochen R. HirschMMMarkus MeyerHMHans‐Jürgen Mägert

Discussion

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Overview

Extends natriuretic peptide signaling to human renal epithelia; leaves open in vivo cardiorenal translation.

Structured PICO

P
Population
Immortalized human kidney epithelial (IHKE-1) cells derived from proximal tubules
I
Intervention
Natriuretic peptides (ANP 10 nM, BNP 10 nM, URO 16 nM, CNP 10 nM)
C
Comparator
Baseline/control conditions (and presence of inhibitors like Ba2+ or genistein)
O
Outcome
Intracellular cGMP levels and membrane voltage (Vm) depolarizationsurrogate

Natriuretic peptides regulate electrogenic transport processes in human kidney epithelial cells via both cGMP-dependent and -independent pathways.

Cite This Study

Hirsch et al. (1999) studied this question. Natriuretic peptides (ANP, BNP, URO, CNP) was evaluated on Membrane voltage (Vm) depolarization and cGMP levels. Natriuretic peptides regulate electrogenic transport processes via cGMP-dependent (ANP, BNP, URO) and cGMP-independent (CNP) pathways that depolarize the membrane voltage of human kidney epithelial cells.

synapsesocial.com/papers/6a979edb618eaafe842d2845https://doi.org/10.1681/asn.v103472
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Receptors of Atrial Natriuretic Factor1992 · 300 citations
  2. 2A new generation of Ca2+ indicators with greatly improved fluorescence properties.1985 · 21,790 citations
  3. 3Atrial natriuretic peptide enhances activity of potassium conductance in adrenal glomerulosa cells1994 · 44 citations
  4. 4A catalogue of splice junction sequences1982 · 3,877 citations
  5. 5Na+ -Dependent and -Independent Amino Acid Transport Systems in Immortalized Human Kidney Epithelial Cells Derived from the Proximal Tubule1998 · 8 citations