Key result
TAT peptide-modified molecular beacons coupled with flow cytometry enabled the rapid detection of poliovirus-infected cells within 30 minutes, detecting as few as 1% infected cells.
Tat peptide-modified molecular beacons coupled with flow cytometry provide a rapid, sensitive, and automated method for detecting viral infections in vitro.
Hypothesis-generating for rapid viral detection assays; leaves open translation beyond in vitro animal models to clinical use.
Rapid and efficient detection of viral infection is crucial for the prevention of disease spread during an outbreak and for timely clinical management. In this paper, the utility of Tat peptide-modified molecular beacons (MBs) as a rapid diagnostic tool for the detection of virus-infected cells was demonstrated. The rapid intracellular delivery mediated by the Tat peptide enabled the detection of infected cells within 30 s, reaching saturation in signal in 30 min. This rapid detection scheme was coupled with flow cytometry (FC), resulting in an automated, high-throughput method for the identification of virus-infected cells. Because of the 2-order-of-magnitude difference in fluorescence intensity between infected and uninfected cells, as few as 1% infected cells could be detected. Because of its speed and sensitivity, this approach may be adapted for the practical diagnosis of multiple viral infections.
No takes yet. Share an insight, caveat, or question.
Sivaraman et al. (2012) studied Poliovirus infection (in vitro). TAT peptide-modified molecular beacons with flow cytometry vs. Uninfected cells was evaluated on Detection of virus-infected cells. TAT peptide-modified molecular beacons coupled with flow cytometry enabled the rapid detection of poliovirus-infected cells within 30 minutes, detecting as few as 1% infected cells.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: