Key result
In Sprague-Dawley rats, a high-salt diet decreased renalase expression, which was not reversed by enalapril or valsartan despite reductions in proteinuria and RAS components.
Why the study?
Does treatment with renin-angiotensin system inhibitors affect the renal expression of renalase in Sprague-Dawley rats fed a high-salt diet?
Does treatment with renin-angiotensin system inhibitors affect the renal expression of renalase in Sprague-Dawley rats fed a high-salt diet?
High salt intake reduces renal expression of renalase, and this process appears independent of the local renin-angiotensin system in rats.
RAS blockade fails to restore renalase in high-salt rat kidneys; leaves open renalase as a distinct therapeutic target beyond RAS inhibition.
BACKGROUND/AIMS: The aim of our study was to investigate the effect of high-salt diet on the renal expression of renalase and the potential role of the local renin-angiotensin system in this process. METHODS: Sprague-Dawley (SD) rats were divided into groups according to salt content in diet and drug treatment as follows: normal-salt diet (NS), high-salt diet (HS), high-salt intake with hydralazine (HS+H), high-salt diet with enalapril (HS+E), and high-salt diet with valsartan (HS+V). The dietary intervention and drugs were given for four weeks. Renin activity and angiotensin II type 1 receptor (AT1R) levels were detected by real-time PCR. Renalase mRNA and protein were also measured. RESULTS: After four weeks, systolic blood pressure and proteinuria were significantly increased in the HS group with respect to the NS group. Dietary salt intake caused a dramatic decrease in renalase expression in the rat kidneys. Renal cortex renin and AT1R increased significantly in the HS and HS+H groups. Urinary protein was positively correlated with renal renin and AT1R levels. However, in the HS+E and HS+V groups, enalapril and valsartan failed to influence renal renalase expression but abolished the increase in proteinuria, renal cortex renin, and AT1R levels with respect to the HS group. CONCLUSION: This study indicates that high salt intake reduces renal expression, and renal RAS may be not involved in the regulation of renalase in SD rats fed with high-salt diet.
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Wang et al. (2015) studied High-salt diet induced renal changes. High-salt diet with enalapril or valsartan vs. Normal-salt diet and high-salt diet alone was evaluated on Renal expression of renalase. In Sprague-Dawley rats, a high-salt diet decreased renalase expression, which was not reversed by enalapril or valsartan despite reductions in proteinuria and RAS components.
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