Key result
A VP6 gene-specific RT-PCR assay was developed and validated for the sensitive and rapid detection of avian group D rotavirus in poultry.
Development of a specific RT-PCR assay for detecting avian group D rotavirus in poultry.
May aid rapid avian group D rotavirus detection in poultry; leaves open field validation and routine adoption.
Development of VP6 Gene Specific Reverse Transcription(RT)-PCR Assay for Detection of Avian Group D Rotavirus in Diarrheic Chickens The group D rotavirus (RVD) is an evolving viral cause of acute gastroenteritis in avian species world over. Despite the increase in the frequency of avian RVD infections world over, isolates from Indian poultry have yet to be characterised. Paucity of epidemiological studies with unavailability of sensitive and rapid detection methods for RVD in poultry led to design of the present study, aiming at development and validation of a RT-PCR assay, targeting the conserved group specific region of VP6 gene of RVD, for virus detection purpose.
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Jobin Jose Kattoor (2014) studied Avian group D rotavirus in diarrheic chickens. VP6 Gene Specific Reverse Transcription(RT)-PCR Assay was evaluated on Detection of avian group D rotavirus. A VP6 gene-specific RT-PCR assay was developed and validated for the sensitive and rapid detection of avian group D rotavirus in poultry.
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