What is the clinical evidence from this study?

Study design: Other. Population: Bleomycin-induced pulmonary fibrosis. Intervention: Macrophage conditioned medium (BAM-CM) vs. Basal medium. Primary outcome: Fibroblast collagen production ([3H]hydroxyproline production, dpm x 10^-4/μg DNA) (42.2% decrease, p=<0.05).

December 1, 1983Journal of Clinical InvestigationOpen Access

Bleomycin-induced pulmonary fibrosis in hamsters. An alveolar macrophage product increases fibroblast prostaglandin E2 and cyclic adenosine monophosphate and suppresses fibroblast proliferation and collagen production.

Q: What are the key findings of this study?

Conditioned medium from alveolar macrophages significantly suppressed fibroblast collagen production and proliferation by stimulating endogenous prostaglandin E2 and cAMP formation.

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Key result

Conditioned medium from alveolar macrophages significantly suppressed fibroblast collagen production and proliferation by stimulating endogenous prostaglandin E2 and cAMP formation.

Population

10-wk-old male Syrian hamsters with bleomycin-induced pulmonary fibrosis and human fetal lung fibroblasts

Comparison

Conditioned medium from bronchoalveolar… vs Conditioned medium from BAM of age-matched…

Design

Preclinical

Follow-up

1-3 weeks

Authors

JCJ G ClarkFred Hutch Cancer Center KKKayla KostalUniversity of British Columbia BMBonaventura MarinoSt. Eugenio Hospital

Discussion

Member takes

Implication

Hypothesis-generating for macrophage-PGE2 antifibrotic signaling in lung fibrosis; leaves open translation to human disease.

Structured PICO

Population

In vitro and animal study using 10-week-old male Syrian hamsters and human fetal lung fibroblasts to evaluate the effect of alveolar macrophage products on fibroblast proliferation and collagen production.

Intervention

Conditioned medium from bronchoalveolar macrophages (BAM) obtained 1-3 weeks after intratracheal bleomycin instillation

Comparator

Conditioned medium from BAM of age-matched, uninjected control hamsters, or basal medium

Outcome

Fibroblast proliferation (measured by [3H]thymidine incorporation) and collagen production (measured by [3H]hydroxyproline production)surrogate

Main Result

Effect estimate: 42.2% decrease

Absolute Event Rate: 1.86% vs 3.21%

p-value: p=<0.05

Alveolar macrophages release a factor that stimulates fibroblast PGE2 and cAMP, suppressing collagen production and potentially limiting collagen accumulation in bleomycin-induced pulmonary fibrosis.

Limitations

In vivo interactions between mononuclear phagocyte products and fibroblasts remain uncertain.
The precise role of serum in the in vitro cultures is not fully known.

Cite This Study

Clark et al. (1983) studied Bleomycin-induced pulmonary fibrosis. Macrophage conditioned medium (BAM-CM) vs. Basal medium was evaluated on Fibroblast collagen production ([3H]hydroxyproline production, dpm x 10^-4/μg DNA) (42.2% decrease, p=<0.05). Conditioned medium from alveolar macrophages significantly suppressed fibroblast collagen production and proliferation by stimulating endogenous prostaglandin E2 and cAMP formation.

synapsesocial.com/papers/6a97e3e655a55e94cce35544 https://doi.org/10.1172/jci111173

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Bleomycin-induced pulmonary fibrosis in hamsters. An alveolar macrophage product increases fibroblast prostaglandin E2 and cyclic adenosine monophosphate and suppresses fibroblast proliferation and collagen production.

Discussion

Member takes

Implication

Structured PICO

Main Result

Limitations

Cite This Study

Bleomycin-induced pulmonary fibrosis in hamsters. An alveolar macrophage product increases fibroblast prostaglandin E2 and cyclic adenosine monophosphate and suppresses fibroblast proliferation and collagen production.

Discussion

Member takes

Implication

Structured PICO

Main Result

Limitations

Cite This Study

Also Consider

Also Consider