Key result
Spa2p directly interacts with Bni1p, and both Spa2p and Rho1p are required for the proper localization of Bni1p at the bud site to regulate the actin cytoskeleton in Saccharomyces cerevisiae.
Population
Saccharomyces cerevisiae (yeast) cells
Comparison
Genetic mutations and protein interaction analysis vs Wild-type cells
Design
Preclinical
Authors
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No direct clinical implications; leaves open whether Spa2p-Rho1p-Bni1p mechanisms are conserved in human cells.
Demonstrates that Rho1p and Spa2p regulate the localization of Bni1p at growth sites to control actin cytoskeleton reorganization in yeast.
Fujiwara et al. (1998) studied Saccharomyces cerevisiae cell polarity and actin cytoskeleton regulation. Genetic mutation of SPA2 and BNI1 vs. Wild-type Saccharomyces cerevisiae was evaluated on Protein interaction and localization. Spa2p directly interacts with Bni1p, and both Spa2p and Rho1p are required for the proper localization of Bni1p at the bud site to regulate the actin cytoskeleton in Saccharomyces cerevisiae.