What question did this study set out to answer?

To investigate how the SH3 domain-containing protein Bem1p and the PH domain-bearing proteins Boi1p and Boi2p interact with Rho-type GTPases during bud emergence in Saccharomyces cerevisiae.

May 15, 1996The Journal of Cell BiologyOpen Access

Associations among PH and SH3 domain-containing proteins and Rho-type GTPases in Yeast.

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To investigate how the SH3 domain-containing protein Bem1p and the PH domain-bearing proteins Boi1p and Boi2p interact with Rho-type GTPases during bud emergence in Saccharomyces cerevisiae.
Assayed physical and two-hybrid interactions between Bem1p, Boi1p, Boi2p, and the Rho-type GTPase Cdc42p.
Evaluated yeast viability, morphogenesis, and bud emergence using single and double deletion mutants of BOI1 and BOI2 as well as gene overexpression constructs for BOI1, CDC42, and RHO3.
Bem1p bound Boi1p and Boi2p through its second SH3 domain, and simultaneous deletion of BOI1 and BOI2 resulted in impaired morphogenesis and reduced viability.
A functional PH domain-containing segment of Boi1p interacted with Cdc42p in a two-hybrid assay, and inhibition of bud emergence caused by Boi1p overexpression was neutralized by co-overexpression of Cdc42p.
Increased expression of the Rho-type GTPase Rho3p rescued bud growth defects in boi1 boi2 mutants, pointing to a functional role for Boi1p and Boi2p in coordinating Cdc42p and Rho3p activity.

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Implication

Experimental study demonstrates functional interactions between Boi1p/Boi2p and Rho-type GTPases in yeast, indicating a coordinated mechanism for cell polarity and bud emergence.

Cite This Study

Bender et al. (1996) studied this question.

synapsesocial.com/papers/6aa4e173fc0ec4e314f2d517 https://doi.org/10.1083/jcb.133.4.879

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Implication

Experimental study demonstrates functional interactions between Boi1p/Boi2p and Rho-type GTPases in yeast, indicating a coordinated mechanism for cell polarity and bud emergence.

Associations among PH and SH3 domain-containing proteins and Rho-type GTPases in Yeast.

Key Points

Discussion

Implication

Cite This Study

Associations among PH and SH3 domain-containing proteins and Rho-type GTPases in Yeast.

Key Points

Discussion

Implication

Cite This Study

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