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When bovine spermatozoa were stored in 20% egg yolk-glycerol extenders buffered with .15, .2, and .25 ~ Tris (hydroxymethyl) aminomethane and adjusted with citric acid to pH's of 6.50, 6.75, and 7.00 optimum sperm survival was found in the 0.2 ~ extender with a pH of 6.75. After eight days of storage at 5 C the motile sperm in this extender averaged 42%, compared with 35% in CUE and 18% in a citrate-yolk-glycerol extender used routinely for freezing bovine spermatozoa.
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Davis et al. (1963) studied this question.
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