May 28, 1996Proceedings of the National Academy of SciencesOpen Access

The rat extracellular superoxide dismutase dimer is converted to a tetramer by the exchange of a single amino acid.

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Population

Rat and mouse genomic DNA/cDNA, and Chinese hamster ovary cells for recombinant expression

Comparison

Site-directed mutagenesis and chimeric… vs Wild-type rat, mouse, and human EC-SODs

Design

Preclinical

Authors

LCLena CarlssonNorthwestern University SMStefan L. MarklundUmeå University HEHelena EdlundUmeå University

Discussion

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Implication

Species-specific EC-SOD variants may guide oxidative-stress models; leaves open relevance to human heparin binding or cardiovascular disease.

Structured PICO

Population

Rat (Sprague-Dawley) and mouse (129/SV) genomic DNA/cDNA, and Chinese hamster ovary (CHO-K1) cells for recombinant expression

Intervention

Site-directed mutagenesis and chimeric constructs of EC-SOD (specifically Asp28Val in rat EC-SOD and Val28Asp in mouse EC-SOD)

Comparator

Wild-type rat, mouse, and human EC-SODs

Outcome

Oligomeric state (molecular weight via size exclusion chromatography) and heparin affinity (via heparin-Sepharose chromatography)surrogate

A single amino acid substitution is responsible for the unique dimeric structure and low heparin affinity of rat extracellular superoxide dismutase compared to the tetrameric form found in other mammals.

Cite This Study

Carlsson et al. (1996) studied this question.

synapsesocial.com/papers/6a9819441119407bb8fac635 https://doi.org/10.1073/pnas.93.11.5219

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Implication

Species-specific EC-SOD variants may guide oxidative-stress models; leaves open relevance to human heparin binding or cardiovascular disease.

The rat extracellular superoxide dismutase dimer is converted to a tetramer by the exchange of a single amino acid.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

The rat extracellular superoxide dismutase dimer is converted to a tetramer by the exchange of a single amino acid.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider