The ratio of serum Angiopoietin-1 to Angiopoietin-2 in patients with cervical cancer is a valuable diagnostic and prognostic biomarker

Figures

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  Figure 1. sAng-2 concentration and sAng-1/sAng-2 ratio are altered in patients with cervical neoplasia. sAng-1 and sAng-2 concentrations in 43 patients of normal control, 44 CIN patients, and 77 cervical cancer patients (62 CSCC patients and 15 CADC patients) were determined by ELISA. The differences in Ang-1 (A), sAng-2 (B), and the sAng-1/sAng-2 ratio (C) between groups are shown.

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  Figure 2. sAng-2 concentration and the sAng-1/sAng-2 ratio are valuable diagnostic biomarkers for cervical lesions. The ROC curves of sAng-2 for discriminating CSCC from normal (A), CSCC from CIN (B), CSCC from normal and CIN (C), CIN and CSCC from normal (D), and invasive cervical cancer from normal (E). The cutoff values of sAng-2 and corresponding sensitivity and specificity to distinguish CSCC from normal are shown (F). The ROC curves of sAng-1/sAng-2 for discriminating CIN from normal (G), CSCC from normal (H), CIN and CSCC from normal (I) and invasive cervical cancer from normal (J). The cutoff values of sAng-1/ sAng-2 and corresponding sensitivity and specificity to distinguish CSCC from normal are listed (K).

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  Figure 3. sAng-2 concentration positively relates with Ang-2 expression on the epithelia and MVD in cervical tissues. Representative immunohistochemical staining of Ang-1 (A), Ang-2 (B) and CD34 (C) in 25 cervical cancer tissue specimens and 10 normal controls. Black arrows denote positively stained epithelial cells, whereas red arrows denote positively staining endothelial cells, all appearing brown. Scale bar, 20 µm. (D) sAng-2 is significantly higher in the patients with positive Ang-2 expression on cervix epithelia than those with negative Ang-2 expression. The scatter diagrams show the correlations of sAng-1 (E), sAng-2 (F) and sAng-1/ sAng-2 ratio (G) to MVD in the 35 cervical tissue specimens.

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  Figure 4. High sAng-1/sAng-2 ratio predicts poorer survival in cervical cancer patients. Kaplan–Meier survival analysis of the progression-free survival and the overall survival among 66 cervical cancer patients stratified by sAng-1 (A, B), sAng-2 (C, D) and sAng-1/sAng-2 ratio (E, F).