Key result
A novel naked-eye colorimetric immunoassay detected Enterovirus 71 with a limit of detection of 0.65 ng/mL, outperforming commercial ELISA and matching PCR diagnostic outcomes in clinical samples.
Why the study?
Does the proposed naked-eye immunoassay provide sensitive and accurate detection of EV71 compared to ELISA and PCR?
Does the proposed naked-eye immunoassay provide sensitive and accurate detection of EV71 compared to ELISA and PCR?
A novel naked-eye immunoassay for EV71 detection demonstrates high sensitivity (LOD 0.65 ng/mL) and comparable diagnostic accuracy to PCR, offering a promising tool for resource-constrained settings.
Development of sensitive, convenient, and cost-effective virus detection product is of great significance to meet the growing demand of clinical diagnosis at the early stage of virus infection. Herein, a naked-eye readout of immunoassay by means of virion bridged catalase-mediated in situ reduction of gold ions and growth of nanoparticles, has been successfully proposed for rapid visual detection of Enterovirus 71 (EV71). Through tailoring the morphologies of the produced gold nanoparticles (GNPs) varying between dispersion and aggregation, a distinguishing color changing was ready for observation. This colorimetric detection assay, by further orchestrating the efficient magnetic enrichment and the high catalytic activity of enzyme, is managed to realize highly sensitive detection of EV71 virions with the limit of detection (LOD) down to 0.65 ng/mL. Our proposed method showed a much lower LOD value than the commercial ELISA for EV71 virion detection. Comparing to the current clinical gold standard polymerase chain reaction (PCR) method, our strategy provided the same diagnostic outcomes after testing real clinical samples. Besides, this strategy has no need of complicated sample pretreatment or expensive instruments. Our presented naked-eye immunoassay method holds a promising prospect for the early detection of virus-infectious disease especially in resource-constrained settings.
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Xiong et al. (2017) studied Enterovirus 71 (EV71) infection. Naked-eye colorimetric immunoassay vs. Commercial ELISA and PCR was evaluated on Limit of detection (LOD) for EV71 virions. A novel naked-eye colorimetric immunoassay detected Enterovirus 71 with a limit of detection of 0.65 ng/mL, outperforming commercial ELISA and matching PCR diagnostic outcomes in clinical samples.
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