Role of Optimizing Transplantation Environmental Conditions in Improving the Survival Rate of Tissue-Cultured Seedlings of Anoectochilus roxburghii (Wall.) Lindl.

Anoectochilus roxburghii (Wall.)Lindl. is highly valued in traditional medicine due to its various pharmacological activities.However, the low survival rate of tissue cultured seedlings after transplantation remains one of the main bottlenecks in achieving large-scale cultivation.This study focuses on the key environmental factors that affect the success of transplanting A. roxburghii flowers, such as light intensity, substrate composition, and mycorrhizal symbiosis.The results indicate that the blue red combination (BR) LED light source plays an important role in promoting seedling growth and flavonoid accumulation, which helps to enhance its medicinal value.Maintaining a suitable temperature and humidity environment can effectively alleviate stress during transplantation and enhance the adaptability of plants.The use of a specific ratio of substrate mixture can improve root development and substrate water retention performance, and increase the survival rate after transplantation.The study also pointed out that inoculation with specific mycorrhizal fungi (such as Ceratobasidium sp.AR2) can enhance the nutrient absorption and stress resistance of plants, further improving the colonization effect.This study provides a scientific basis for optimizing the transplanting conditions of A. roxburghii, which is helpful for its sustainable cultivation and resource protection, and provides useful references for the transplanting management of other medicinal plants.