Biochemical analysis isolates and characterizes epithiospecifier protein in Brassica napus seeds, suggesting involvement in jasmonate-mediated plant defense.
Key Points
To isolate and characterize the epithiospecifier protein from Brassica napus seeds and assess its sequence identity with known plant defense proteins.
Purified the epithiospecifier protein from Brassica napus seeds using anion-exchange and gel filtration chromatography.
Determined molecular weight and isoelectric point, and sequenced tryptic peptides covering approximately 50% of the total protein sequence for homology analysis.
Purified epithiospecifier protein exhibited an apparent molecular weight of approximately 39 kDa and an isoelectric point (pI) of 5.3.
Sequence analysis of tryptic peptides demonstrated 81% identity with a hypothetical 37 kDa protein and several jasmonate-inducible proteins from Arabidopsis thaliana.