Key result
IgG fouling shifted the capture location of 20 nm gold nanoparticles further upstream within the Viresolve® Pro filter, but had no measurable effect in the Viresolve® NFP membrane.
Why the study?
Although membrane fouling can affect virus filter retention, direct measurements of how protein fouling alters the location of virus capture within these membranes were lacking.
Population
Viresolve Pro and Viresolve NFP membranes tested with gold nanoparticles
Comparison
Protein fouling by human IgG vs no fouling across different nanoparticle sizes
Design
In vitro laboratory evaluation using scanning electron microscopy
Authors
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Informs virus filter selection for consistent clearance in bioprocessing; leaves open validation in scaled manufacturing.
Protein fouling by IgG alters the capture location of small (20 nm) nanoparticles in specific virus filters (Viresolve Pro), providing insights into virus retention characteristics during biotherapeutic production.
Fallahianbijan et al. (2019) studied this question. Protein fouling by human immunoglobulin G (IgG) was evaluated on Location of virus capture within membranes using gold nanoparticles. IgG fouling shifted the capture location of 20 nm gold nanoparticles further upstream within the Viresolve® Pro filter, but had no measurable effect in the Viresolve® NFP membrane.
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