What question did this study set out to answer?

To elucidate the mechanisms by which therapeutic proteins and membrane fouling diminish virus retention during biotherapeutic filtration.

November 23, 2023Biotechnology and BioengineeringOpen Access

Impact of proteins and protein fouling on virus retention during virus removal filtration

View Full Paper

Ask AI

Bookmark

Authors

MAMohammad A. AfzalPennsylvania State University AZAndrew L. ZydneyPennsylvania State University

Discussion

Member takes

Implication

Laboratory study demonstrates that protein fouling reduces parvovirus retention by over 100-fold during membrane filtration, highlighting critical challenges in bioprocess viral clearance.

Key Points

To elucidate the mechanisms by which therapeutic proteins and membrane fouling diminish virus retention during biotherapeutic filtration.
Conducted filtration challenges using a single layer of a commercial Pegasus SV4 virus filter.
Used bacteriophage ФX174 as a model parvovirus alongside human immunoglobulin (hIgG) and bovine serum albumin (BSA) in clean and pre-fouled filter setups.
Analyzed virus transport and capture dynamics using an internal polarization model.
Addition of 5 g/L hIgG caused a greater than 100-fold reduction in virus retention compared to protein-free controls.
Membranes pre-fouled with hIgG similarly reduced ФX174 retention.
Internal polarization modeling indicated that hIgG nearly eliminated irreversible viral capture while accelerating the release of previously retained viruses.

Cite This Study

Afzal et al. (2023) studied this question.

synapsesocial.com/papers/6a9864c6030cd346144d7cff https://doi.org/10.1002/bit.28607

View Full Paper

Ask AI

Bookmark

Implication

Laboratory study demonstrates that protein fouling reduces parvovirus retention by over 100-fold during membrane filtration, highlighting critical challenges in bioprocess viral clearance.

Impact of proteins and protein fouling on virus retention during virus removal filtration

Discussion

Member takes

Implication

Key Points

Cite This Study

Impact of proteins and protein fouling on virus retention during virus removal filtration

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider