What question did this study set out to answer?

To quantitatively assess how filtrate flux variations and process interruptions influence the virus retention performance of a homogeneous polyvinylidene fluoride membrane filter.

April 5, 2022Biotechnology Progress

Effect of filtrate flux and process disruptions on virus retention by a relatively homogeneous virus removal membrane

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Authors

MAMohammad A. AfzalPennsylvania State University AZAndrew L. ZydneyPennsylvania State University

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Implication

Experimental study demonstrates reduced virus retention during low flux and operational disruptions in membrane filters, indicating critical risks for bioprocess safety.

Key Points

To quantitatively assess how filtrate flux variations and process interruptions influence the virus retention performance of a homogeneous polyvinylidene fluoride membrane filter.
Measured virus retention performance across a homogeneous polyvinylidene fluoride (PVDF) virus removal filter using bacteriophage ϕX174 as a model virus.
Evaluated the impact of variable filtrate flux rates alongside process disruptions of varying duration and timing, fitting retention behavior to an internal polarization model.
Virus retention declined as filtrate flux decreased and showed a continuous slight decline across the duration of the filtration run.
Process disruptions reduced post-disruption virus retention by up to a factor of 1000 (3-log reduction), with retention loss governed by the timing and duration of the pause.
Virus accumulation and release dynamics were quantitatively captured by an internal polarization model with flux-dependent transport parameters.

Cite This Study

Afzal et al. (2022) studied this question.

synapsesocial.com/papers/6a97c034ce42e14121e1759c https://doi.org/10.1002/btpr.3255

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Implication

Experimental study demonstrates reduced virus retention during low flux and operational disruptions in membrane filters, indicating critical risks for bioprocess safety.

Effect of filtrate flux and process disruptions on virus retention by a relatively homogeneous virus removal membrane

Discussion

Member takes

Implication

Key Points

Cite This Study

Effect of filtrate flux and process disruptions on virus retention by a relatively homogeneous virus removal membrane

Discussion

Member takes

Implication

Key Points

Cite This Study

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