What is the clinical evidence from this study?

Intervention: Pressure release. Primary outcome: Retention of bacteriophage ΦX174.

What question did this study set out to answer?

To evaluate the effect of a temporary transmembrane pressure release on bacteriophage ΦX174 retention and internal capture mechanics within the Ultipor DV20 virus filter.

September 11, 2013Biotechnology and Bioengineering

Effects of a pressure release on virus retention with the Ultipor DV20 membrane

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Key result

A temporary release in transmembrane pressure caused a significant transient increase (typically by an order of magnitude) in bacteriophage ΦX174 transmission through the Ultipor DV20 membrane.

Population

Ultipor DV20 membrane challenged with bacteriophage ΦX174

Design

Preclinical

Authors

MWMelissa A. WoodsPennsylvania State University AZAndrew L. ZydneyPennsylvania State University

Discussion

Member takes

Implication

May increase virus breakthrough risk during filtration pressure fluctuations; hypothesis-generating for membrane process controls.

Key Points

To evaluate the effect of a temporary transmembrane pressure release on bacteriophage ΦX174 retention and internal capture mechanics within the Ultipor DV20 virus filter.
Conducted challenge tests with bacteriophage ΦX174 on Ultipor DV20 membranes under conditions of temporary transmembrane pressure release.
Visualized bacteriophage capture and redistribution across the filter depth using confocal microscopy and modeled transport with a modified internal polarization model.
Detected a significant transient spike in phage transmission, typically by an order of magnitude, immediately following the pressure release.
Confirmed that the temporary increase in virus transmission resulted from trapped bacteriophages migrating deeper into the filter rather than structural membrane damage or fluid back-flow.

Structured PICO

Population

Ultipor DV20 membrane challenged with bacteriophage ΦX174

Intervention

Temporary release in transmembrane pressure

Outcome

Virus retention/transmission

A temporary release in transmembrane pressure causes a significant transient increase in virus transmission through the Ultipor DV20 membrane due to migration of captured viruses.

Cite This Study

Woods et al. (2013) studied this question. Pressure release was evaluated on Retention of bacteriophage ΦX174. A temporary release in transmembrane pressure caused a significant transient increase (typically by an order of magnitude) in bacteriophage ΦX174 transmission through the Ultipor DV20 membrane.

synapsesocial.com/papers/6a985a45e847acbecd245a97 https://doi.org/10.1002/bit.25112

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Effects of a pressure release on virus retention with the Ultipor DV20 membrane

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Effects of a pressure release on virus retention with the Ultipor DV20 membrane

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider