What is the clinical evidence from this study?

Intervention: Ultipor VF Grade DV20 virus filters. Primary outcome: Retention of PP7 and PR772 bacteriophage.

What question did this study set out to answer?

To elucidate the mechanisms responsible for the decline in virus retention during high-throughput filtration through Ultipor VF Grade DV20 membrane filters.

March 10, 2014Biotechnology Progress

Internal virus polarization model for virus retention by the Ultipor® VF Grade DV20 membrane

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Key result

Filtration of the small PP7 phage through Ultipor VF Grade DV20 virus filters showed a 2-log decline in retention at high throughputs, which was well described by an internal polarization model.

Population

PP7 and PR772 bacteriophages and Ultipor VF Grade DV20 virus filters

Design

Preclinical

Authors

NJNigel B. JacksonPall Corporation (United States)MBMeisam BakhshayeshiBiogen (United States)AZAndrew L. ZydneyPennsylvania State University

Discussion

Member takes

Implication

Supports virus filter validation recommendations; leaves open generalizability from phage models to bioprocessing.

Key Points

To elucidate the mechanisms responsible for the decline in virus retention during high-throughput filtration through Ultipor VF Grade DV20 membrane filters.
Evaluated the retention of small PP7 and large PR772 bacteriophages across Ultipor VF Grade DV20 filters under constant pressure filtration, including conditions with immunoglobulin G membrane prefouling.
Developed an internal polarization model describing phage accumulation within the filter's upstream reservoir layer and validated phage distribution using confocal microscopy of fluorescently labeled phages.
Large PR772 bacteriophages were fully retained under all test conditions, whereas small PP7 phages exhibited a 2-log decline in retention at high throughput despite no decrease in filtrate flux.
Prefouling DV20 membranes with an immunoglobulin G solution did not alter bacteriophage retention.
The internal polarization model showed close agreement with retention data across multiple phage titers, confirming that viral accumulation in the upper membrane layer increases challenge to the lower rejection layer.

Structured PICO

Population

PP7 and PR772 bacteriophages and Ultipor VF Grade DV20 virus filters

Intervention

Constant pressure filtration; prefouling the membrane with an immunoglobulin G solution

Outcome

Virus retentionsurrogate

An internal polarization model explains the throughput-dependent decline in virus retention by membrane filters, supporting current recommendations for virus retention validation studies.

Cite This Study

Jackson et al. (2014) studied this question. Ultipor VF Grade DV20 virus filters was evaluated on Retention of PP7 and PR772 bacteriophage. Filtration of the small PP7 phage through Ultipor VF Grade DV20 virus filters showed a 2-log decline in retention at high throughputs, which was well described by an internal polarization model.

synapsesocial.com/papers/6a985a45e847acbecd245a96 https://doi.org/10.1002/btpr.1897

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Internal virus polarization model for virus retention by the Ultipor® VF Grade DV20 membrane

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Internal virus polarization model for virus retention by the Ultipor® VF Grade DV20 membrane

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider