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January 24, 2020AJP Heart and Circulatory PhysiologyOpen Access

Cardiac sympathetic nerve transdifferentiation reduces action potential heterogeneity after myocardial infarction

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Key result

Deletion of choline acetyltransferase from noradrenergic neurons increased action potential duration dispersion after myocardial infarction compared to wild-type mice (11.9 vs. 8.2 ms; P<0.05).

Why the study?

Cardiac sympathetic nerves undergo cholinergic transdifferentiation after reperfused MI, but the functional electrophysiological consequences have never been explored.

Population

WT mice and choline acetyltransferase KO mice undergoing MI or sham surgery

Comparison

KO MI vs WT MI

Design

Ex vivo animal experimental study with optical mapping

Authors

LWLianguo WangAOAntoinette OlivasSSSamantha D. Francis Stuart

Discussion

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Overview

May reduce post-MI arrhythmogenic substrate via sympathetic cholinergic transdifferentiation in mice; leaves open translation to human arrhythmia risk.

Structured PICO

P
Population
Adult wild-type and noradrenergic choline acetyltransferase knockout mice undergoing myocardial infarction or sham surgery.
I
Intervention
Deletion of choline acetyltransferase from adult noradrenergic neurons (KO model) preventing cholinergic transdifferentiation post-MI.
C
Comparator
Wild-type (WT) mice with intact cholinergic transdifferentiation post-MI.
O
Outcome
Electrophysiological activity assessed with optical mapping of action potentials (AP) and intracellular Ca2+ transients (CaT) in innervated Langendorff-perfused hearts.surrogate

Main Result

Absolute Event Rate: 11.9% vs 8.2%

p-value: p=<0.05

Cholinergic transdifferentiation of cardiac sympathetic neurons following myocardial infarction reduces action potential duration dispersion, suggesting a potential endogenous antiarrhythmic mechanism.

Cite This Study

Wang et al. (2020) studied Myocardial infarction (n=14). Deletion of choline acetyltransferase from adult noradrenergic neurons vs. Wild-type mice was evaluated on Action potential duration dispersion (p=<0.05). Deletion of choline acetyltransferase from noradrenergic neurons increased action potential duration dispersion after myocardial infarction compared to wild-type mice (11.9 vs. 8.2 ms; P<0.05).

synapsesocial.com/papers/6a98664a2dec5628dce02abchttps://doi.org/10.1152/ajpheart.00412.2019
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