January 1, 1996Biochemistry

The Plasma and Cytoplasmic Forms of Human Gelsolin Differ in Disulfide Structure

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Population

Human plasma and cytoplasmic gelsolin, and recombinant human plasma gelsolin expressed in Escherichia coli…

Design

Preclinical

Authors

DWDingyi WenChina University of Geosciences (Beijing)KCKaren CorinaBiogen (United States)ECE P ChowBiogen (United States)

Discussion

Member takes

Implication

Plasma gelsolin's domain 2 disulfide distinguishes it from cytoplasmic form; leaves open recombinant folding issues for research applications.

Structured PICO

Population

Human plasma and cytoplasmic gelsolin, and recombinant human plasma gelsolin expressed in Escherichia coli and Cos green monkey cells

Intervention

Biochemical analysis including cysteine-specific modification with 4-vinylpyridine, HPLC peptide mapping, mass spectrometry, and limited proteolysis

Outcome

Disulfide structure differences between plasma and cytoplasmic gelsolinsurrogate

Intracellular and extracellular gelsolins are structurally distinct due to a disulfide bond in domain 2 of the plasma form, suggesting some recombinant preparations may be improperly folded.

Cite This Study

Wen et al. (1996) studied this question.

synapsesocial.com/papers/6a9866e29279e0cbcb9776fb https://doi.org/10.1021/bi960920n

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Implication

Plasma gelsolin's domain 2 disulfide distinguishes it from cytoplasmic form; leaves open recombinant folding issues for research applications.

The Plasma and Cytoplasmic Forms of Human Gelsolin Differ in Disulfide Structure

Discussion

Member takes

Implication

Structured PICO

Cite This Study

The Plasma and Cytoplasmic Forms of Human Gelsolin Differ in Disulfide Structure

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider