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September 1, 1999RNAOpen Access

A stable hairpin preceded by a short open reading frame promotes nonlinear ribosome migration on a synthetic mRNA leader

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Population

In vitro and in vivo translation models

Comparison

Artificial mRNA leader with a stable stem… vs Wild-type CaMV 35S RNA 600-nt leader and various…

Design

Preclinical

Authors

MHMaja Hemmings-MieszczakNovartis (Switzerland)THThomas HöhnKarlsruhe Institute of Technology

Discussion

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Implication

Synthetic leaders enable compact shunting for efficient translation; leaves open mammalian therapeutic mRNA applications.

Structured PICO

P
Population
In vitro (wheat germ extract) and in vivo (transiently transfected Orychophragmus violaceus plant protoplasts) translation models
I
Intervention
Artificial mRNA leader with a stable stem flanked by unstructured sequences derived from CaMV 35S RNA leader
C
Comparator
Wild-type CaMV 35S RNA 600-nt leader and various mutated versions
O
Outcome
Translation efficiency of a downstream CAT-reporter genesurrogate

A synthetic mRNA leader with a low-energy stem preceded by a short uORF can efficiently support translation via nonlinear ribosome scanning, mimicking the viral leader.

Cite This Study

Hemmings-Mieszczak et al. (1999) studied this question.

synapsesocial.com/papers/6a9870fab4ff0464b2ad8ef2https://doi.org/10.1017/s1355838299990325
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Also Consider

Synapse has enriched 3 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Selective translation initiation by ribosome jumping in adenovirus-infected and heat-shocked cells.1996 · 193 citations
  2. 2Circumstances and Mechanisms of Inhibition of Translation by Secondary Structure in Eucaryotic mRNAs1989 · 254 citations
  3. 3Role of Translocation in the Activation and Function of Protein Kinase B1997 · 1,060 citations