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Method validation study demonstrates precise quantification of vitamin E isomers in walnuts, highlighting an effective chromatographic protocol for food quality assessment.
A high performance liquid chromatographic (HPLC) method for the determination of tocopherols and tocotrienols in walnut samples is described. The compounds were extracted with n‐hexane, using a simple solid‐liquid extraction procedure. Tocol was used as internal standard and BHT as anti‐oxidant. The chromatographic separation was achieved using an Inertsil 5 SI normal phase column operating with isocratic elution of n‐hexane/1,4‐dioxane (96.5∶3.5, v/v), at a flow rate of 0.7 mL/min. The effluent was monitored by a series arrangement of a diode‐array detector followed by a fluorescence detector. The detection limits were low, between 0.037 and 0.266 µg/mL. The method was precise (% CV less than 2.8%), accurate (% CV less than 5.6%), and, as a general rule, the recovery values were high (mean values ranging from 93.4% to 104.0%).
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Amaral et al. (2005) studied this question.
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