Titanium platelet-rich fibrin as doxycycline delivery system: An in vitro study

Background: Locally delivered anti-infective agents are one of the frequently used treatment strategies for the treatment of periodontal disease. Doxycycline (Doxy) is frequently preferred in periodontal therapy. Various carrier systems are available such as collagen sponges and mucoadhesive polymers. The use of platelet-rich fibrin (PRF) is a good strategy for drug delivery systems with its three-dimensional matrix structure as a scaffold, as it can naturally degrade within a certain period without causing any allergic/inflammatory conditions in the body. Titanium PRF (T-PRF) has a denser fibrin structure than PRF with a long resorption time and may be a good candidate for long-term drug delivery and release. Aim: The study aimed to study and compare the physical and antibacterial properties of T-PRF and Doxy-loaded T-PRF. Materials and Methods: It is an in vitro study. The T-PRF and T-PRF loaded with Doxy as T-PRF/Doxy will be prepared and their physical properties such as length and consistency will be evaluated. Antibacterial activities against Staphylococcus aureus and Pseudomonas aeruginosa will be investigated by measuring the zone of inhibition after 24 h and 48 h. Results and Conclusion: T-PRF loaded with Doxy can be used as a local drug delivery agent. However, very little literature is available in this field. And in vivo , studies are required to determine the efficiency in periodontitis and peri-implantitis cases.