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June 7, 2019International Journal of Molecular SciencesOpen Access

Role of Platelet Glycoprotein VI and Tyrosine Kinase Syk in Thrombus Formation on Collagen-Like Surfaces

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Key result

Syk inhibition with PRT-060318 suppressed platelet activation and aggregation on collagen-like structures, indicating GPVI-dependent signaling through Syk supports thrombus formation.

Why the study?

To determine the Syk-mediated thrombogenic activity of several collagen peptides and fibrillar type I and III collagens.

Does Syk inhibition with PRT-060318 reduce thrombus formation on collagen-like surfaces in whole blood and platelet suspensions?

Population

Whole blood and platelet suspensions

Comparison

Syk inhibitor PRT-060318 vs control across various collagen surfaces

Design

In vitro experimental perfusion study

Authors

NJNatalie J. JoossISIlaria De SimoneIPIsabella Provenzale

Discussion

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Overview

Syk inhibition may attenuate thrombus formation in preclinical models; leaves open translation to human antithrombotic therapy.

Structured PICO

Does Syk inhibition with PRT-060318 reduce thrombus formation on collagen-like surfaces in whole blood and platelet suspensions?

P
Population
Whole blood and platelet suspensions (in vitro model)
I
Intervention
Syk inhibitor PRT-060318
O
Outcome
Thrombus formation (assessed by platelet adhesion, activation, aggregation, and contraction) and cytosolic Ca2+ risessurrogate

GPVI-dependent signaling through Syk supports platelet activation in thrombus formation on collagen-like structures regardless of the presence of a (GPO)n sequence.

Cite This Study

Jooss et al. (2019) studied Thrombus formation. Syk inhibitor PRT-060318 was evaluated on Thrombus formation (platelet adhesion, activation, aggregation, and contraction). Syk inhibition with PRT-060318 suppressed platelet activation and aggregation on collagen-like structures, indicating GPVI-dependent signaling through Syk supports thrombus formation.

synapsesocial.com/papers/6a9882afc8b3ba98c080d99fhttps://doi.org/10.3390/ijms20112788
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Role of Tyrosine Kinase Syk in Thrombus Stabilisation at High Shear2022 · 14 citations
  2. 2Proplatelet formation is selectively inhibited by collagen type I through Syk-independent GPVI signaling2016 · 54 citations
  3. 3Roles of Focal Adhesion Kinase PTK2 and Integrin αIIbβ3 Signaling in Collagen- and GPVI-Dependent Thrombus Formation under Shear2022 · 7 citations
  4. 4Inhibition of Src but not Syk causes weak reversal of GPVI-mediated platelet aggregation measured by light transmission aggregometry2022 · 4 citations
  5. 5Platelets undergo phosphorylation of Syk at Y525/526 and Y352 in response to pathophysiological shear stress2008 · 31 citations