Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
April 9, 2003FEBS Letters

A PLCγ2‐independent platelet collagen aggregation requiring functional association of GPVI and integrin α 2 β 1

View Full Paper
Ask AI
Bookmark
Share

Key result

PLC gamma 2-null platelets showed a markedly reduced residual aggregation response to collagen fibres compared to wild-type (6% vs 74%), with minimal granule secretion and no shape change.

Why the study?

Does PLCgamma2 knockout alter platelet activation and aggregation in response to collagen in mice?

Population

PLC gamma 2 -/- mice and wild-type mice

Comparison

Genetic knockout of PLC gamma 2 and exposure to… vs Wild-type mice

Design

Preclinical

Authors

PMPierre ManginCNChristelle NonneAEAnita Eckly

Discussion

Loading...

Member takes

Overview

Reveals PLCγ2-independent collagen responses in mice; leaves open translation to human platelet function and antithrombotic strategies.

Structured PICO

Does PLCgamma2 knockout alter platelet activation and aggregation in response to collagen in mice?

P
Population
PLC gamma 2 -/- mice evaluated for platelet activation and aggregation responses.
E
Exposure
Genetic knockout of PLC gamma 2 and exposure to various platelet agonists/antagonists (collagen fibres, convulxin, collagen-related peptide, antibodies against integrin alpha2beta1 and GPVI, aspirin, ADP receptor blockade)
C
Comparator
Wild-type mice
O
Outcome
Platelet aggregation response, granule secretion, and shape change in response to collagen and other agonistssurrogate

Main Result

Absolute Event Rate: 6% vs 74%

The study identifies a PLCgamma2-independent collagen activation pathway in platelets requiring cooperation between GPVI and integrin alpha2beta1.

Cite This Study

Mangin et al. (2003) studied Platelet activation. PLC gamma 2 deficiency vs. Wild-type mice was evaluated on Platelet aggregation response to collagen fibres. PLC gamma 2-null platelets showed a markedly reduced residual aggregation response to collagen fibres compared to wild-type (6% vs 74%), with minimal granule secretion and no shape change.

synapsesocial.com/papers/6a9882afc8b3ba98c080d9a8https://doi.org/10.1016/s0014-5793(03)00337-5
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Distinct roles of GPVI and integrin α<sub>2</sub>β<sub>1</sub> in platelet shape change and aggregation induced by different collagens2002 · 86 citations
  2. 2A Critical Role for Phospholipase Cγ2 in αIIbβ3-mediated Platelet Spreading2003 · 132 citations
  3. 3Reciprocal Signaling by Integrin and Nonintegrin Receptors during Collagen Activation of Platelets2003 · 97 citations
  4. 4Differential integrin activity mediated by platelet collagen receptor engagement under flow conditions2017 · 27 citations
  5. 5Platelet-collagen interaction: is GPVI the central receptor?2003 · 1,163 citations